GPU in PCIEX4 instead of x16

This video explains everything you need to know about PCIe slots, including PCIe slot

While not optimal, what happens if you put the Startech card into PCIE_4? Will it work then even though your GFX card will only be at 8X? Merely

candelas de los muertos senorita suerte slot PCIegen 4with up to 2x bandwidth 7PCIe slots Up to300Wauxiliary power One PCIe x4 gen 3 Apple IO card Four PCIe x8 gen 4 Two single height Two double

candy blitz slot but since im using the first pci-express x16 slot with graphic card i can only use the second slot and second pci-express x16 slot conforms